The intracellular plasma membrane-connected compartment in the assembly of HIV-1 in human macrophages.

ABSTRACT:

Background

In HIV-infected macrophages, newly formed progeny virus particles accumulate in intracellular plasma membrane-connected compartments (IPMCs). Although the virus is usually seen in these compartments, it is unclear whether HIV assembly is specifically targeted to IPMCs or whether some viruses may also form at the cell surface but are not detected, as particles budding from the latter site will be released into the medium.

Results

To investigate the fidelity of HIV-1 targeting to IPMCs compared to the cell surface directly, we generated mutants defective in recruitment of the Endosomal Sorting Complexes Required for Transport (ESCRT) proteins required for virus scission. For mutants unable to bind the ESCRT-I component Tsg101, HIV release was inhibited and light and

SUBMITTER: Nkwe DO 

PROVIDER: S-EPMC4919869 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature