Viral infection causes a shift in the self peptide repertoire presented by human MHC class I molecules.

ABSTRACT:

Purpose

MHC class I presentation of peptides allows T cells to survey the cytoplasmic protein milieu of host cells. During infection, presentation of self peptides is, in part, replaced by presentation of microbial peptides. However, little is known about the self peptides presented during infection, despite the fact that microbial infections alter host cell gene expression patterns and protein metabolism.

Experimental design

The self peptide repertoire presented by HLA-A*01;01, HLA-A*02;01, HLA-B*07;02, HLA-B*35;01, and HLA-B*45;01 (where HLA is human leukocyte antigen) was determined by tandem MS before and after vaccinia virus infection.

Results

We observed a profound alteration in the self peptide repertoire with hundreds of self peptides uniquely presented after

SUBMITTER: Spencer CT 

PROVIDER: S-EPMC4920078 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature