Peptide sequences from the first Castoroides ohioensis skull and the utility of old museum collections for palaeoproteomics.

ABSTRACT: Vertebrate fossils have been collected for hundreds of years and are stored in museum collections around the world. These remains provide a readily available resource to search for preserved proteins; however, the vast majority of palaeoproteomic studies have focused on relatively recently collected bones with a well-known handling history. Here, we characterize proteins from the nasal turbinates of the first Castoroides ohioensis skull ever discovered. Collected in 1845, this is the oldest museum-curated specimen characterized using palaeoproteomic tools. Our mass spectrometry analysis detected many collagen I peptides, a peptide from haemoglobin beta, and in vivo and diagenetic post-translational modifications. Additionally, the identified collagen I sequences provide enough resolution t

SUBMITTER: Cleland TP 

PROVIDER: S-EPMC4920319 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature