In Vivo Non-Invasive Tracking of Macrophage Recruitment to Experimental Stroke.

ABSTRACT: Brain-infiltrating monocyte-derived macrophages are one of the key players in the local immune response after stroke. It is now widely accepted that the inflammatory response is not an exclusively destructive process. However, the underlying molecular mechanisms needed for proper regulation still remain to be elucidated. Here, we propose an in vitro labelling strategy for multimodal in vivo observation of macrophage dynamics distinguished from brain-residing microglia response. Prior to intracerebral transplantation into the striatum of recipient mice or systemic administration, monocytes and macrophages, isolated from luciferase-expressing mice, were labelled with superparamagnetic iron oxide particles. Temporo-spatial localization was monitored by magnetic resonance imaging, whereas surv

SUBMITTER: Selt M 

PROVIDER: S-EPMC4920382 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature