An In Silico Approach for Identification of the Pathogenic Species, Helicobacter pylori and Its Relatives.

ABSTRACT: Helicobacter is an economically important genus within the phylum Proteobacteria and include many species which cause many diseases in humans. With the conventional methods, it is difficult to identify them easily due to the high genetic similarity among its species. In the present study, 361 16S rRNA (rrs) gene sequences belonging to 45 species of genus Helicobacter were analyzed. Out of these, 264 sequences of 10 clinically relevant species (including Helicobacter pylori) were used. rrs gene sequences were analyzed to obtain a phylogenetic framework tree, in silico restriction enzyme analysis and species-specific conserved motifs. Protein sequences of another housekeeping gene, hsp60 were also subjected to phylogenetic analysis to supplement the data obtained using rrs sequences. Using t

SUBMITTER: Puri A 

PROVIDER: S-EPMC4920758 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature