Filamentation of Metabolic Enzymes in Saccharomyces cerevisiae.

ABSTRACT: Compartmentation via filamentation has recently emerged as a novel mechanism for metabolic regulation. In order to identify filament-forming metabolic enzymes systematically, we performed a genome-wide screening of all strains available from an open reading frame-GFP collection in Saccharomyces cerevisiae. We discovered nine novel filament-forming proteins and also confirmed those identified previously. From the 4159 strains, we found 23 proteins, mostly metabolic enzymes, which are capable of forming filaments in vivo. In silico protein-protein interaction analysis suggests that these filament-forming proteins can be clustered into several groups, including translational initiation machinery and glucose and nitrogen metabolic pathways. Using glutamine-utilising enzymes as examples, we fou

SUBMITTER: Shen QJ 

PROVIDER: S-EPMC4920916 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature