AFBI assay - Aptamer Fluorescence Binding and Internalization assay for cultured adherent cells.

ABSTRACT: The SELEX (Systematic Evolution of Ligands by Exponential Enrichment) process allows for the enrichment of DNA or RNA aptamers from a complex nucleic acid library that are specific for a target molecule. The SELEX process has been adapted from identifying aptamers in vitro using recombinant target protein to cell-based methodologies (Cell-SELEX), where the targets are expressed on the surface of cells. One major advantage of Cell-SELEX is that the target molecules are maintained in a native confirmation. Additionally, Cell-SELEX may be used to discover novel therapeutic biomarkers by performing selections on diseased versus healthy cells. However, a caveat to Cell-SELEX is that testing of single aptamers identified in the selection is laborious, time-consuming, and expensive. The most freq

SUBMITTER: Thiel WH 

PROVIDER: S-EPMC4921262 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature