Identification and characterization of EGF receptor in individual exosomes by fluorescence-activated vesicle sorting.

ABSTRACT: Exosomes are small, 40-130 nm secreted extracellular vesicles that recently have become the subject of intense focus as agents of intercellular communication, disease biomarkers and potential vehicles for drug delivery. It is currently unknown whether a cell produces different populations of exosomes with distinct cargo and separable functions. To address this question, high-resolution methods are needed. Using a commercial flow cytometer and directly labelled fluorescent antibodies, we show the feasibility of using fluorescence-activated vesicle sorting (FAVS) to analyse and sort individual exosomes isolated by sequential ultracentrifugation from the conditioned medium of DiFi cells, a human colorectal cancer cell line. EGFR and the exosomal marker, CD9, were detected on individual DiFi e

SUBMITTER: Higginbotham JN 

PROVIDER: S-EPMC4921784 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature