Autonomous interconversion between adult pancreatic α-cells and β-cells after differential metabolic challenges.

ABSTRACT:

Background

Evidence hints at the ability of β-cells to emerge from non-β-cells upon genetic or pharmacological interventions. However, their quantitative contributions to the process of autonomous β-cell regeneration without genetic or pharmacological manipulations remain to be determined.

Methods & results

Using PANIC-ATTAC mice, a model of titratable, acute β-cell apoptosis capable of autonomous, and effective islet mass regeneration, we demonstrate that an extended washout of residual tamoxifen activity is crucial for β-cell lineage tracing studies using the tamoxifen-inducible Cre/loxP systems. We further establish a doxycycline-inducible system to label different cell types in the mouse pancreas and pursued a highly quantitative assessment to trace adult β-cells after v

SUBMITTER: Ye R 

PROVIDER: S-EPMC4921793 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature