AGPAT2 is essential for postnatal development and maintenance of white and brown adipose tissue.

ABSTRACT:

Objective

Characterize the cellular and molecular events responsible for lipodystrophy in AGPAT2 deficient mice.

Methods

Adipose tissue and differentiated MEF were assessed using light and electron microscopy, followed by protein (immunoblots) and mRNA analysis (qPCR). Phospholipid profiling was determined by electrospray ionization tandem mass spectrometry (ESI-MS/MS).

Results

In contrast to adult Agpat2 (-/-) mice, fetuses and newborn Agpat2 (-/-) mice have normal mass of white and brown adipose tissue. Loss of both the adipose tissue depots occurs during the first week of postnatal life as a consequence of adipocyte death and inflammatory infiltration of the adipose tissue. At the ultrastructural level, adipose tissue of newborn Agpat2 (-/-) mice is virtually devoi

SUBMITTER: Cautivo KM 

PROVIDER: S-EPMC4921804 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature