Matrix metalloproteinase-9-dependent mechanisms of reduced contractility and increased stiffness in the aging heart.

ABSTRACT:

Purpose

Matrix metalloproteinases (MMPs) collectively degrade all extracellular matrix (ECM) proteins. Of the MMPs, MMP-9 has the strongest link to the development of cardiac dysfunction. Aging associates with increased MMP-9 expression in the left ventricle (LV) and reduced cardiac function. We investigated the effect of MMP-9 deletion on the cardiac ECM in aged animals.

Experimental design

We used male and female middle-aged (10- to16-month old) and old (20- to 24-month old) wild-type (WT) and MMP-9 null mice (n = 6/genotype/age). LVs were decellularized to remove highly abundant mitochondrial proteins that could mask identification of relative lower abundant components, analyzed by shotgun proteomics, and proteins of interest validated by immunoblot.

Results

Elasti

SUBMITTER: Padmanabhan Iyer R 

PROVIDER: S-EPMC4922304 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature