Enhanced Islet Cell Nucleomegaly Defines Diffuse Congenital Hyperinsulinism in Infancy but Not Other Forms of the Disease.

ABSTRACT:

Objectives

To quantify islet cell nucleomegaly in controls and tissues obtained from patients with congenital hyperinsulinism in infancy (CHI) and to examine the association of nucleomegaly with proliferation.

Methods

High-content analysis of histologic sections and serial block-face scanning electron microscopy were used to quantify nucleomegaly.

Results

Enlarged islet cell nuclear areas were 4.3-fold larger than unaffected nuclei, and the mean nuclear volume increased to approximately threefold. Nucleomegaly was a normal feature of pediatric islets and detected in the normal regions of the pancreas from patients with focal CHI. The incidence of nucleomegaly was highest in diffuse CHI (CHI-D), with more than 45% of islets containing two or more affected cells. While

SUBMITTER: Han B 

PROVIDER: S-EPMC4922485 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature