Large-scale imaging of cortical dynamics during sensory perception and behavior.

ABSTRACT: Sensory-driven behaviors engage a cascade of cortical regions to process sensory input and generate motor output. To investigate the temporal dynamics of neural activity at this global scale, we have improved and integrated tools to perform functional imaging across large areas of cortex using a transgenic mouse expressing the genetically encoded calcium sensor GCaMP6s, together with a head-fixed visual discrimination behavior. This technique allows imaging of activity across the dorsal surface of cortex, with spatial resolution adequate to detect differential activity in local regions at least as small as 100 μm. Imaging during an orientation discrimination task reveals a progression of activity in different cortical regions associated with different phases of the task. After cortex-wide

SUBMITTER: Wekselblatt JB 

PROVIDER: S-EPMC4922607 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature