Identification of Human Immunodeficiency Virus Type-1 Subtypes by Heteroduplex Mobility Assay.

ABSTRACT:

Background

Human immunodeficiency virus type-1 (HIV-1) has developed marked genomic sequence differences over the course of an epidemic because of an error prone reverse transcriptase (RT), which rapidly incorporates mutations resulting in genomic diversity, altered cell tropism, immune escape and variable resistance to antiretroviral drugs. The best preventive strategy for HIV control is development of an efficacious prophylactic vaccine using the most appropriate (antigenically related) subtypes. On the basis of phylogenetic analysis, HIV strains can be separated into major group "M" consisting of genetic subtypes A-K, "N", the new group and "O", the outlier group.

Methods

Heteroduplex mobility assay (HMA) is a rapid, economical and reliable technique of subtyping HIV-1. I

SUBMITTER: Sahni AK 

PROVIDER: S-EPMC4922754 | biostudies-literature | 2007 Jul

REPOSITORIES: biostudies-literature