Embryological Origin of Human Smooth Muscle Cells Influences Their Ability to Support Endothelial Network Formation.

ABSTRACT:

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Vascular smooth muscle cells (SMCs) from distinct anatomic locations derive from different embryonic origins. Here we investigated the respective potential of different embryonic origin-specific SMCs derived from human embryonic stem cells (hESCs) to support endothelial network formation in vitro. SMCs of three distinct embryological origins were derived from an mStrawberry-expressing hESC line and were cocultured with green fluorescent protein-expressing human umbilical vein endothelial cells (HUVECs) to investigate the effects of distinct SMC subtypes on endothelial network formation. Quantitative analysis demonstrated that lateral mesoderm (LM)-derived SMCs best supported HUVEC network complexity and survival in three-dimensional coculture in Matrigel. The effects of

SUBMITTER: Bargehr J 

PROVIDER: S-EPMC4922852 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature