Autofluorescence as a Signal to Sort Developing Glandular Trichomes by Flow Cytometry.

ABSTRACT: The industrial relevance of a number of metabolites produced in plant glandular trichomes (GTs) has spurred research on these specialized organs for a number of years. Most of the research, however, has focused on the elucidation of secondary metabolite pathways and comparatively little has been undertaken on the development and differentiation of GTs. One way to gain insight into these developmental processes is to generate stage-specific transcriptome and metabolome data. The difficulty for this resides in the isolation of early stages of development of the GTs. Here we describe a method for the separation and isolation of intact young and mature type VI trichomes from the wild tomato species Solanum habrochaites. The final and key step of the method uses cell sorting based on distinct a

SUBMITTER: Bergau N 

PROVIDER: S-EPMC4923063 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature