Epigenetic repression of ribosomal RNA transcription by ROCK-dependent aberrant cytoskeletal organization.

ABSTRACT: It is known that ribosomal RNA (rRNA) synthesis is regulated by cellular energy and proliferation status. In this study, we investigated rRNA gene transcription in response to cytoskeletal stress. Our data revealed that the cell shape constrained by isotropic but not elongated micropatterns in HeLa cells led to a significant reduction in rRNA transcription dependent on ROCK. Expression of a dominant-active form of ROCK also repressed rRNA transcription. Isotropic constraint and ROCK over-activation led to different types of aberrant F-actin organization, but their suppression effects on rRNA transcription were similarly reversed by inhibition of histone deacetylase (HDAC) or overexpression of a dominant negative form of Nesprin, which shields the signal transmitted from actin filament to t

SUBMITTER: Wu TH 

PROVIDER: S-EPMC4923894 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature