Simultaneous purification of DNA and RNA from microbiota in a single colonic mucosal biopsy.

ABSTRACT:

Background

Nucleic acid purification methods are of importance when performing microbiota studies and especially when analysing the intestinal microbiota as we here find a wide range of different microbes. Various considerations must be taken to lyse the microbial cell wall of each microbe. In the present article, we compare several tissue lysis steps and commercial purification kits, to achieve a joint RNA and DNA purification protocol for the purpose of investigating the microbiota and the microbiota-host interactions in a single colonic mucosal tissue sample.

Results

A further optimised tissue homogenisation and lysis protocol comprising mechanical bead beating, lysis buffer replacement and enzymatic treatment, in combination with the AllPrep DNA/RNA Mini Kit (Qiagen, Hil

SUBMITTER: Moen AE 

PROVIDER: S-EPMC4924232 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature