Quantification and Classification of E. coli Proteome Utilization and Unused Protein Costs across Environments.

ABSTRACT: The costs and benefits of protein expression are balanced through evolution. Expression of un-utilized protein (that have no benefits in the current environment) incurs a quantifiable fitness costs on cellular growth rates; however, the magnitude and variability of un-utilized protein expression in natural settings is unknown, largely due to the challenge in determining environment-specific proteome utilization. We address this challenge using absolute and global proteomics data combined with a recently developed genome-scale model of Escherichia coli that computes the environment-specific cost and utility of the proteome on a per gene basis. We show that nearly half of the proteome mass is unused in certain environments and accounting for the cost of this unused protein expression explain

SUBMITTER: O'Brien EJ 

PROVIDER: S-EPMC4924638 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature