Accurate Measurement of the Effects of All Amino-Acid Mutations on Influenza Hemagglutinin.

ABSTRACT: Influenza genes evolve mostly via point mutations, and so knowing the effect of every amino-acid mutation provides information about evolutionary paths available to the virus. We and others have combined high-throughput mutagenesis with deep sequencing to estimate the effects of large numbers of mutations to influenza genes. However, these measurements have suffered from substantial experimental noise due to a variety of technical problems, the most prominent of which is bottlenecking during the generation of mutant viruses from plasmids. Here we describe advances that ameliorate these problems, enabling us to measure with greatly improved accuracy and reproducibility the effects of all amino-acid mutations to an H1 influenza hemagglutinin on viral replication in cell culture. The largest

SUBMITTER: Doud MB 

PROVIDER: S-EPMC4926175 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature