Efficient production of multi-modified pigs for xenotransplantation by 'combineering', gene stacking and gene editing.

ABSTRACT: Xenotransplantation from pigs could alleviate the shortage of human tissues and organs for transplantation. Means have been identified to overcome hyperacute rejection and acute vascular rejection mechanisms mounted by the recipient. The challenge is to combine multiple genetic modifications to enable normal animal breeding and meet the demand for transplants. We used two methods to colocate xenoprotective transgenes at one locus, sequential targeted transgene placement - 'gene stacking', and cointegration of multiple engineered large vectors - 'combineering', to generate pigs carrying modifications considered necessary to inhibit short to mid-term xenograft rejection. Pigs were generated by serial nuclear transfer and analysed at intermediate stages. Human complement inhibitors CD46, CD55

SUBMITTER: Fischer K 

PROVIDER: S-EPMC4926246 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature