Development of EST Intron-Targeting SNP Markers for Panax ginseng and Their Application to Cultivar Authentication.

ABSTRACT: Panax ginseng is one of the most valuable medicinal plants in the Orient. The low level of genetic variation has limited the application of molecular markers for cultivar authentication and marker-assisted selection in cultivated ginseng. To exploit DNA polymorphism within ginseng cultivars, ginseng expressed sequence tags (ESTs) were searched against the potential intron polymorphism (PIP) database to predict the positions of introns. Intron-flanking primers were then designed in conserved exon regions and used to amplify across the more variable introns. Sequencing results showed that single nucleotide polymorphisms (SNPs), as well as indels, were detected in four EST-derived introns, and SNP markers specific to "Gopoong" and "K-1" were first reported in this study. Based on cultivar-spe

SUBMITTER: Wang H 

PROVIDER: S-EPMC4926418 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature