Optimization and comparison of bottom-up proteomic sample preparation for early-stage Xenopus laevis embryos.

ABSTRACT: Xenopus laevis is an important model organism in developmental biology. While there is a large literature on changes in the organism's transcriptome during development, the study of its proteome is at an embryonic state. Several papers have been published recently that characterize the proteome of X. laevis eggs and early-stage embryos; however, proteomic sample preparation optimizations have not been reported. Sample preparation is challenging because a large fraction (~90 % by weight) of the egg or early-stage embryo is yolk. We compared three common protein extraction buffer systems, mammalian Cell-PE LB(TM) lysing buffer (NP40), sodium dodecyl sulfate (SDS), and 8 M urea, in terms of protein extraction efficiency and protein identifications. SDS extracts contained the highest concentra

SUBMITTER: Peuchen EH 

PROVIDER: S-EPMC4926613 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature