Comprehensive identification of host modulators of HIV-1 replication using multiple orthologous RNAi reagents.

ABSTRACT: RNAi screens have implicated hundreds of host proteins as HIV-1 dependency factors (HDFs). While informative, these early studies overlap poorly due to false positives and false negatives. To ameliorate these issues, we combined information from the existing HDF screens together with new screens performed with multiple orthologous RNAi reagents (MORR). In addition to being traditionally validated, the MORR screens and the historical HDF screens were quantitatively integrated by the adaptation of an established analysis program, RIGER, for the collective interpretation of each gene's phenotypic significance. False positives were addressed by the removal of poorly expressed candidates through gene expression filtering, as well as with GESS, which identifies off-target effects. This workflow

SUBMITTER: Zhu J 

PROVIDER: S-EPMC4926641 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature