Proteomics analyses of subcutaneous adipocytes reveal novel abnormalities in human insulin resistance.

ABSTRACT:

Objective

To provide a more global view of adipocyte changes in human insulin resistance by proteomics analyses.

Methods

Baseline biopsies of abdominal subcutaneous adipose tissue were obtained from 23 subjects without diabetes. Euglycemic clamps were used to divide subjects into an insulin-resistant group (IR, N = 10) and an insulin-sensitive (IS, N = 13) group, which were of similar age and gender but unequal adiposity (greater in IR). Proteins of isolated adipocytes were quantified by mass spectrometry using normalized spectral abundance factors.

Results

Of 1,245 proteins assigned, 30 were detected in at least 12 of the 23 subjects that differed significantly in abundance ≥1.5-fold between IR and IS. IR displayed a pattern of increased cytoskeletal proteins and dec

SUBMITTER: Xie X 

PROVIDER: S-EPMC4926648 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature