Combination of Controlled Release Platelet-Rich Plasma Alginate Beads and Bone Morphogenetic Protein-2 Genetically Modified Mesenchymal Stem Cells for Bone Regeneration.

ABSTRACT:

Background

Platelet-rich plasma (PRP) consists of platelet-derived growth factor and transforming growth factor-β that increase proliferation of mesenchymal stem cells (MSCs), whereas bone morphogenetic protein-2 (BMP2) promotes osteogenic differentiation of MSCs. However, the high degradation rate of fibrin leads to the dissociation of cytokines even before the process of bone regeneration begins. To the best of the authors' knowledge, this is the first study to examine the combined effect of sustained release of PRP from alginate beads on BMP2-modified MSC osteogenic differentiation in vitro and sustained release of PRP alone on a fracture defect model ex vivo as well as its effect on calvarial suture closure.

Methods

After optimizing the alginate concentration for microsp

SUBMITTER: Fernandes G 

PROVIDER: S-EPMC4927087 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature