Optimization of a Membrane Feeding Assay for Plasmodium vivax Infection in Anopheles albimanus.

ABSTRACT:

Introduction

Individuals exposed to malaria infections for a long time develop immune responses capable of blocking Plasmodium transmission to mosquito vectors, potentially limiting parasite spreading in nature. Development of a malaria TB vaccine requires a better understanding of the mechanisms and main effectors responsible for transmission blocking (TB) responses. The lack of an in vitro culture system for Plasmodium vivax has been an important drawback for development of a standardized method to assess TB responses to this parasite. This study evaluated host, vector, and parasite factors that may influence Anopheles mosquito infection in order to develop an efficient and reliable assay to assess the TB immunity.

Methods/principal findings

A total of 94 P. vivax infected

SUBMITTER: Vallejo AF 

PROVIDER: S-EPMC4927173 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature