Evaluation of bacterial diversity recovered from petroleum samples using different physical matrices.

ABSTRACT: Unraveling the microbial diversity and its complexity in petroleum reservoir environments has been a challenge throughout the years. Despite the techniques developed in order to improve methodologies involving DNA extraction from crude oil, microbial enrichments using different culture conditions can be applied as a way to increase the recovery of DNA from environments with low cellular density for further microbiological analyses. This work aimed at the evaluation of different matrices (arenite, shale and polyurethane foam) as support materials for microbial growth and biofilm formation in enrichments using a biodegraded petroleum sample as inoculum in sulfate reducing condition. Subsequent microbial diversity characterization was carried out using Scanning Electronic Microscopy (SEM), De

SUBMITTER: Dellagnezze BM 

PROVIDER: S-EPMC4927652 | biostudies-literature | 2016 Jul-Sep

REPOSITORIES: biostudies-literature