Huntington's Disease Protein Huntingtin Associates with its own mRNA.

ABSTRACT:

Background

The Huntington's disease (HD) protein huntingtin (Htt) plays a role in multiple cellular pathways. Deregulation of one or more of these pathways by the mutant Htt protein has been suggested to contribute to the disease pathogenesis. Our recent discovery-based proteomics studies have uncovered RNA binding proteins and translation factors associated with the endogenous Htt protein purified from mouse brains, suggesting a potential new role for Htt in RNA transport and translation.

Objective

To investigate how Htt might affect RNA metabolism we set out to purify and analyze RNA associated with Htt.

Methods

RNA was extracted from immunopurified Htt-containing protein complexes and analyzed by microarrays and RNA-Seq.

Results

Surprisingly, the most enrich

SUBMITTER: Culver BP 

PROVIDER: S-EPMC4927879 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature