Transcriptomic Profiling of Extracellular RNAs Present in Cerebrospinal Fluid Identifies Differentially Expressed Transcripts in Parkinson's Disease.

ABSTRACT:

Background

Parkinson's disease (PD) is a debilitating neurological disorder for which prognostic and diagnostic biomarkers are lacking. Cerebrospinal fluid (CSF) is an accessible body fluid that comes into direct contact with the central nervous system (CNS) and acts as a nuclease-free repository where RNA transcripts shed by brain tissues can reside for extended periods of time.

Objective

We studied the RNA species present in the CSF of PD patients to identify novel diagnostic biomarkers.

Methods

Small volumes of CSF from 27 PD patients and 30 healthy age- and sex-matched controls were used for RNA extraction followed by next-generation sequencing (RNA-seq) using the Illumina platform. CSF contains a number of fragmented RNA species that were individually sequenced a

SUBMITTER: Hossein-Nezhad A 

PROVIDER: S-EPMC4927907 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature