Dataset of integrin-linked kinase protein: Protein interactions in cardiomyocytes identified by mass spectrometry.

ABSTRACT: Using hearts from mice overexpressing integrin linked kinase (ILK) behind the cardiac specific promoter αMHC, we have performed immunoprecipitation and mass spectrometry to identify novel ILK protein:protein interactions that regulate cardiomyocyte activity and calcium flux. Integrin linked kinase complexes were captured from mouse heart lysates using a commercial antibody, with subsequent liquid chromatography tandem mass spectral analysis. Interacting partners were identified using the MASCOT server, and important interactions verified using reverse immunoprecipitation and mass spectrometry. All ILK interacting proteins were identified in a non-biased manner, and are stored in the ProteomeXchange Consortium via the PRIDE partner repository (reference ID PRIDE: PXD001053). The functional

SUBMITTER: Traister A 

PROVIDER: S-EPMC4927968 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature