Site-specific bacterial chromosome engineering mediated by IntA integrase from Rhizobium etli.

ABSTRACT:

Background

The bacterial chromosome may be used to stably maintain foreign DNA in the mega-base range. Integration into the chromosome circumvents issues such as plasmid replication, stability, incompatibility, and copy number variance. The site-specific integrase IntA from Rhizobium etli CFN42 catalyzes a direct recombination between two specific DNA sites: attA and attD (23 bp). This recombination is stable. The aim of this work was to develop a R. etli derivative that may be used as recipient for the integration of foreign DNA in the chromosome, adapting the IntA catalyzed site-specific recombination system.

Results

To fulfill our aim, we designed a Rhizobium etli CFN42 derivative, containing a "landing pad" (LP) integrated into the chromosome. The LP sector consists of a

SUBMITTER: Hernandez-Tamayo R 

PROVIDER: S-EPMC4928290 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature