In vivo genome-wide profiling reveals a tissue-specific role for 5-formylcytosine.

ABSTRACT:

Background

Genome-wide methylation of cytosine can be modulated in the presence of TET and thymine DNA glycosylase (TDG) enzymes. TET is able to oxidise 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC). TDG can excise the oxidative products 5fC and 5caC, initiating base excision repair. These modified bases are stable and detectable in the genome, suggesting that they could have epigenetic functions in their own right. However, functional investigation of the genome-wide distribution of 5fC has been restricted to cell culture-based systems, while its in vivo profile remains unknown.

Results

Here, we describe the first analysis of the in vivo genome-wide profile of 5fC across a range of tissues from both wild-type

SUBMITTER: Iurlaro M 

PROVIDER: S-EPMC4928330 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature