Sources of variability in measurements of cardiac troponin T in a community-based sample: the atherosclerosis risk in communities study.

ABSTRACT:

Background

Application of cardiac troponin T (cTnT) as a marker of myocyte damage requires knowledge of its measurement variability. Using a highly sensitive assay for measurement, we evaluated the long-term storage stability of plasma cTnT at -70 °C and the sources of cTnT variability.

Methods

Samples from the Atherosclerosis Risk in Communities study collected in 1996-1998 and 2005-2006 were assayed centrally to quantify variability in cTnT attributable to processing (replicates from same blood draw, n = 87), laboratory (replicates after freeze thaw, n = 29), short-term (n = 40) and long-term biological variation (repeat visit, n = 38), and degradation in frozen storage (n = 7677).

Results

Approximately 30% of this population-based cohort had cTnT concentrations bel

SUBMITTER: Agarwal SK 

PROVIDER: S-EPMC4928588 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature