Proteomic Analysis of Vitreous Humor in Retinal Vein Occlusion.

ABSTRACT:

Purpose

To analyze the protein profile of human vitreous of untreated patients with retinal vein occlusion (RVO).

Methods

Sixty-eight vitreous humor (VH) samples (44 from patients with treatment naïve RVO, 24 controls with idiopathic floaters) were analyzed in this clinical-experimental study using capillary electrophoresis coupled to mass spectrometer and tandem mass spectrometry. To define potential candidate protein markers of RVO, proteomic analysis was performed on RVO patients (n = 30) and compared with controls (n = 16). To determine validity of potential biomarker candidates in RVO, receiver operating characteristic (ROC) was performed by using proteome data of independent RVO (n = 14) and control samples (n = 8).

Results

Ninety-four different proteins (736 tr

SUBMITTER: Reich M 

PROVIDER: S-EPMC4928959 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature