Rhizosecretion improves the production of Cyanovirin-N in Nicotiana tabacum through simplified downstream processing.

ABSTRACT: Rhizosecretion has many advantages for the production of recombinant pharmaceuticals, notably facile downstream processing from hydroponic medium. The aim of this study was to increase yields of the HIV microbicide candidate, Cyanovirin-N (CV-N), obtained using this production platform and to develop a simplified methodology for its downstream processing from hydroponic medium. Placing hydroponic cultures on an orbital shaker more than doubled the concentration of CV-N in the hydroponic medium compared to plants which remained stationary, reaching a maximum of approximately 20μg/ml in one week, which is more than 3 times higher than previously reported yields. The protein composition of the hydroponic medium, the rhizosecretome, was characterised in plants cultured with or without the plan

SUBMITTER: Madeira LM 

PROVIDER: S-EPMC4929045 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature