Ultrasensitive multiplex optical quantification of bacteria in large samples of biofluids.

ABSTRACT: Efficient treatments in bacterial infections require the fast and accurate recognition of pathogens, with concentrations as low as one per milliliter in the case of septicemia. Detecting and quantifying bacteria in such low concentrations is challenging and typically demands cultures of large samples of blood (~1 milliliter) extending over 24-72 hours. This delay seriously compromises the health of patients. Here we demonstrate a fast microorganism optical detection system for the exhaustive identification and quantification of pathogens in volumes of biofluids with clinical relevance (~1 milliliter) in minutes. We drive each type of bacteria to accumulate antibody functionalized SERS-labelled silver nanoparticles. Particle aggregation on the bacteria membranes renders dense arrays of inte

SUBMITTER: Pazos-Perez N 

PROVIDER: S-EPMC4929498 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature