Proteomic profiling of cellular steatosis with concomitant oxidative stress in vitro.

ABSTRACT:

Background

Nutrient excess underpins the development of nonalcoholic fatty liver disease (NAFLD). The ensuing metabolic derangement is characterised by increased cellular respiration, oxidative stress and mitochondrial impairment. We have previously recapitulated these events in an in vitro cellular steatosis model. Here, we examined the distinct patterns of protein expression involved using a proteomics approach.

Methods

Human hepatoblastoma C3A cells were treated with a combination of energy substrates; lactate (L), pyruvate (P), octanoate (O) and ammonia (N). Proteins extracts were trypsinized and analyzed on a capillary HPLC OrbitrapXL mass spectrometer. Proteins were quantified using a label-free intensity based approach. Functional enrichment analysis was performed usi

SUBMITTER: Lockman KA 

PROVIDER: S-EPMC4930558 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature