Accurate Measurement of the in vivo Ammonium Concentration in Saccharomyces cerevisiae.

ABSTRACT: Ammonium (NH₄⁺) is the most common N-source for yeast fermentations, and N-limitation is frequently applied to reduce growth and increase product yields. While there is significant molecular knowledge on NH₄⁺ transport and assimilation, there have been few attempts to measure the in vivo concentration of this metabolite. In this article, we present a sensitive and accurate analytical method to quantify the in vivo intracellular ammonium concentration in Saccharomyces cerevisiae based on standard rapid sampling and metabolomics techniques. The method validation experiments required the development of a proper sample processing protocol to minimize ammonium production/consumption during biomass extraction by assessing the impact of amino acid degradation-an element that is often overlooked.

SUBMITTER: Cueto-Rojas HF 

PROVIDER: S-EPMC4931543 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature