Differential expression of microRNAs in retinal vasculopathy caused by selective Muller cell disruption.

ABSTRACT: Vascular changes and photoreceptor degeneration are features of age-related macular degeneration, diabetic retinopathy and macular telangiectasis. We have profiled the differential expression of microRNAs and analysed their target genes in transgenic mice in which induced Müller cell disruption results in photoreceptor degeneration, vascular leak and deep retinal neovascularisation. We identified 9 miRNAs which were differentially expressed during the development of retinal neovascularization and chose miR-200b and its target genes for further study. Using qRT-PCR and western blot analysis, we found that downregulation of miR-200b was negatively correlated with its target genes, including zinc finger E-box binding homeobox (ZEB) 1 and 2 and vascular endothelial growth factor receptor 1. Do

SUBMITTER: Chung SH 

PROVIDER: S-EPMC4931578 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature