VirusMapper: open-source nanoscale mapping of viral architecture through super-resolution microscopy.

ABSTRACT: The nanoscale molecular assembly of mammalian viruses during their infectious life cycle remains poorly understood. Their small dimensions, generally bellow the 300nm diffraction limit of light microscopes, has limited most imaging studies to electron microscopy. The recent development of super-resolution (SR) light microscopy now allows the visualisation of viral structures at resolutions of tens of nanometers. In addition, these techniques provide the added benefit of molecular specific labelling and the capacity to investigate viral structural dynamics using live-cell microscopy. However, there is a lack of robust analytical tools that allow for precise mapping of viral structure within the setting of infection. Here we present an open-source analytical framework that combines super-res

SUBMITTER: Gray RD 

PROVIDER: S-EPMC4931586 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature