High-throughput dual-colour precision imaging for brain-wide connectome with cytoarchitectonic landmarks at the cellular level.

ABSTRACT: The precise annotation and accurate identification of neural structures are prerequisites for studying mammalian brain function. The orientation of neurons and neural circuits is usually determined by mapping brain images to coarse axial-sampling planar reference atlases. However, individual differences at the cellular level likely lead to position errors and an inability to orient neural projections at single-cell resolution. Here, we present a high-throughput precision imaging method that can acquire a co-localized brain-wide data set of both fluorescent-labelled neurons and counterstained cell bodies at a voxel size of 0.32 × 0.32 × 2.0 μm in 3 days for a single mouse brain. We acquire mouse whole-brain imaging data sets of multiple types of neurons and projections with anatomical annot

SUBMITTER: Gong H 

PROVIDER: S-EPMC4932192 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature