A Presenilin-2-ARF4 trafficking axis modulates Notch signaling during epidermal differentiation.

ABSTRACT: How primary cilia impact epidermal growth and differentiation during embryogenesis is poorly understood. Here, we show that during skin development, Notch signaling occurs within the ciliated, differentiating cells of the first few suprabasal epidermal layers. Moreover, both Notch signaling and cilia disappear in the upper layers, where key ciliary proteins distribute to cell-cell borders. Extending this correlation, we find that Presenilin-2 localizes to basal bodies/cilia through a conserved VxPx motif. When this motif is mutated, a GFP-tagged Presenilin-2 still localizes to intercellular borders, but basal body localization is lost. Notably, in contrast to wild type, this mutant fails to rescue epidermal differentiation defects seen upon Psen1 and 2 knockdown. Screening components impli

SUBMITTER: Ezratty EJ 

PROVIDER: S-EPMC4932368 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature