Mitotic genes are transcriptionally upregulated in the fibroblast irradiated with very low doses of UV-C.

ABSTRACT: Ultraviolet (UV) radiation induces a variety of biological effects, including DNA damage response and cell signaling pathways. We performed transcriptome analysis using microarray in human primary cultured fibroblasts irradiated with UV-C (0.5 or 5 J/m(2)) and harvested at 4 or 12 h following UV exposure. All transcript data were analyzed by comparison with the corresponding results in non-irradiated (control) cells. The number of genes with significantly altered expression (≥2-fold difference relative to the control) is higher in the sample irradiated with high dose of UV, suggesting that gene expression was UV dose-dependent. Pathway analysis on the upregulated genes at 12 h indicates that the expression of some cell cycle-related genes was predominantly induced irrespective of UV-dose.

SUBMITTER: Takeuchi S 

PROVIDER: S-EPMC4932599 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature