The influence of tumor size and environment on gene expression in commonly used human tumor lines.

ABSTRACT:

Background

The expression profiles of solid tumor models in rodents have been only minimally studied despite their extensive use to develop anticancer agents. We have applied RNA expression profiling using Affymetrix U95A GeneChips to address fundamental biological questions about human tumor lines.

Methods

To determine whether gene expression changed significantly as a tumor increased in size, we analyzed samples from two human colon carcinoma lines (Colo205 and HCT-116) at three different sizes (200 mg, 500 mg and 1000 mg). To investigate whether gene expression was influenced by the strain of mouse, tumor samples isolated from C.B-17 SCID and Nu/Nu mice were also compared. Finally, the gene expression differences between tissue culture and in vivo samples were investigate

SUBMITTER: Gieseg MA 

PROVIDER: S-EPMC493269 | biostudies-literature | 2004 Jul

REPOSITORIES: biostudies-literature