Second harmonic generation correlation spectroscopy for characterizing translationally diffusing protein nanocrystals.

ABSTRACT: Second harmonic generation correlation spectroscopy (SHG-CS) is demonstrated as a new approach to protein nanocrystal characterization. A novel line-scanning approach was performed to enable autocorrelation analysis without sample damage from the intense incident beam. An analytical model for autocorrelation was developed, which includes a correction for the optical scattering forces arising when focusing intense, infrared beams. SHG-CS was applied to the analysis of BaTiO3 nanoparticles ranging from 200 to ∼500 nm and of photosystem I nanocrystals. A size distribution was recovered for each sample and compared with the size histogram measured by scanning electron microscopy (SEM). Good agreement was observed between the two independent measurements. The intrinsic selectivity of the second

SUBMITTER: Dow XY 

PROVIDER: S-EPMC4932918 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature