Longitudinal multiparameter assay of lymphocyte interactions from onset by microfluidic cell pairing and culture.

ABSTRACT: Resolving how the early signaling events initiated by cell-cell interactions are transduced into diverse functional outcomes necessitates correlated measurements at various stages. Typical approaches that rely on bulk cocultures and population-wide correlations, however, only reveal these relationships broadly at the population level, not within each individual cell. Here, we present a microfluidics-based cell-cell interaction assay that enables longitudinal investigation of lymphocyte interactions at the single-cell level through microfluidic cell pairing, on-chip culture, and multiparameter assays, and allows recovery of desired cell pairs by micromanipulation for off-chip culture and analyses. Well-defined initiation of interactions enables probing cellular responses from the very onset

SUBMITTER: Dura B 

PROVIDER: S-EPMC4932925 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature