Simultaneous all-optical manipulation and recording of neural circuit activity with cellular resolution in vivo.

ABSTRACT: We describe an all-optical strategy for simultaneously manipulating and recording the activity of multiple neurons with cellular resolution in vivo. We performed simultaneous two-photon optogenetic activation and calcium imaging by coexpression of a red-shifted opsin and a genetically encoded calcium indicator. A spatial light modulator allows tens of user-selected neurons to be targeted for spatiotemporally precise concurrent optogenetic activation, while simultaneous fast calcium imaging provides high-resolution network-wide readout of the manipulation with negligible optical cross-talk. Proof-of-principle experiments in mouse barrel cortex demonstrate interrogation of the same neuronal population during different behavioral states and targeting of neuronal ensembles based on their funct

SUBMITTER: Packer AM 

PROVIDER: S-EPMC4933203 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature