Browse
Submit Data
Databases
API
Help

Dataset Information

5 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Telomere Visualization in Tissue Sections using Pyrrole-Imidazole Polyamide Probes.

ABSTRACT: Pyrrole-Imidazole (PI) polyamides bind to specific DNA sequences in the minor groove with high affinity. Specific DNA labeling by PI polyamides does not require DNA denaturation with harsh treatments of heat and formamide and has the advantages of rapid and less disruptive processing. Previously, we developed tandem hairpin PI polyamide probes (TH59 series), which label telomeres in cultured cell lines more efficiently than conventional methods, such as fluorescence in situ hybridization (FISH). Here, we demonstrate that a TH59 derivative, HPTH59-b, along with immunostaining for specifying cell types in the tissues, visualizes telomeres in mouse and human tissue sections. Quantitative measurements of telomere length with single-cell resolution suggested shorter telomeres in the proliferating cell fractions of tumor than in non-tumor tissues. Thus, PI polyamides are a promising alternative for telomere labeling in clinical research, as well as in cell biology.

SUBMITTER: Sasaki A

PROVIDER: S-EPMC4933941 | biostudies-literature |

REPOSITORIES: biostudies-literature

ACCESS DATA

Json Xml

Similar Datasets

Tandem trimer pyrrole-imidazole polyamide probes targeting 18 base pairs in human telomere sequences.

Project description: Not available

| S-EPMC5645774 | biostudies-literature

Antitumor activity of a pyrrole-imidazole polyamide

Project description:We report that a DNA minor groove binding hairpin pyrrole-imidazole (Py-Im) polyamide interferes with RNA polymerase II (RNAP2) activity in cell culture. Genome-wide mapping of RNAP2 binding shows reduction of occupancy preferentially at transcription start sites (TSS), while occupancy at enhancer sites are is unchanged. Genome-wide mapping of RNA polymerase II in LNCaP cells treated with DHT and DHT with Py-Im polyamide.

2013-01-31 | E-GEOD-43253 | biostudies-arrayexpress

Antitumor activity of a pyrrole-imidazole polyamide.

Project description: Not available

| S-EPMC3562772 | biostudies-literature

Telomere-specific chromatin capture using a pyrrole–imidazole polyamide probe for the identification of proteins and non-coding

Project description:Background: Knowing chromatin components at a DNA regulatory element at any given time is essential for understanding how the element works during cellular proliferation, differentiation and development. A region-specific chromatin purification is an invaluable approach to dissecting the comprehensive chromatin composition at a particular region. Several methods (e.g., PICh, enChIP, CAPTURE and CLASP) have been developed for isolating and analyzing chromatin components. However, all of them have some shortcomings in identifying non-coding RNA associated with DNA regulatory elements. Results: We have developed a new approach for affinity purification of specific chromatin segments employing an N-methyl pyrrole (P)−N-methylimidazole (I) (PI) polyamide probe, which binds to a specific sequence in double-stranded DNA via Watson–Crick base pairing as a minor groove binder. This new technique is called proteomics and RNA-omics of isolated chromatin segments (PI-PRICh). Using PI-PRICh to isolate mouse and human telomeric components, we found enrichments of shelterin proteins, the well-known telomerase RNA component (TERC) and telomeric repeat-containing RNA (TERRA). When PI-PRICh was performed for alternative lengthening of telomere (ALT) cells with highly recombinogenic telomeres, in addition to the conventional telomeric chromatin, we obtained chromatin regions containing telomeric repeat insertions scattered in the genome and their associated RNAs. Conclusion: PI-PRICh reproducibly identified both the protein and RNA components of telomeric chromatin when targeting telomere repeats. PI polyamide is a promising alternative to simultaneously isolate associated proteins and RNAs of sequence-specific chromatin regions under native conditions, allowing better understanding of chromatin organization and functions within the cell.

2021-10-10 | GSE181609 | GEO

Antitumor activity of a pyrrole-imidazole polyamide

2013-01-31 | GSE43253 | GEO

Characterization and solubilization of pyrrole-imidazole polyamide aggregates.

Project description: Not available

| S-EPMC3375050 | biostudies-literature

Telomere-specific chromatin capture using a pyrrole–imidazole polyamide probe for the identification of proteins and non-coding [PI-PRICh, DNA_pulldown1]

2021-10-10 | GSE181608 | GEO

Telomere-specific chromatin capture using a pyrrole–imidazole polyamide probe for the identification of proteins and non-coding [PI-PRICh, RNA_pulldown2]

2021-10-10 | GSE181607 | GEO

Telomere-specific chromatin capture using a pyrrole–imidazole polyamide probe for the identification of proteins and non-coding [PI-PRICh, RNA_pulldown1]

2021-10-10 | GSE181603 | GEO