Single-cell genome-wide bisulfite sequencing uncovers extensive heterogeneity in the mouse liver methylome.

ABSTRACT:

Background

Transmission fidelity of CpG DNA methylation patterns is not foolproof, with error rates from less than 1 to well over 10 % per CpG site, dependent on preservation of the methylated or unmethylated state and the type of sequence. This suggests a fairly high chance of errors. However, the consequences of such errors in terms of cell-to-cell variation have never been demonstrated by experimentally measuring intra-tissue heterogeneity in an adult organism.

Results

We employ single-cell DNA methylomics to analyze heterogeneity of genome-wide 5-methylcytosine (5mC) patterns within mouse liver. Our results indicate a surprisingly high level of heterogeneity, corresponding to an average epivariation frequency of approximately 3.3 %, with regions containing H3K4me1 being

SUBMITTER: Gravina S 

PROVIDER: S-EPMC4934005 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature